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高产反式-4-羟脯氨酸重组大肠杆菌的构建以及发酵条件优化，刘合栋，袁春伟，脯氨酸4-羟化酶可直接将游离的脯氨酸的第四位上加羟基生成4-羟脯氨酸。为了使脯氨酸4-羟化酶基因在重组大肠杆菌中得到高表达，根据
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